Interactions between Hyaluronic Acid, Lysozyme, Peroxidase, and Glucose Oxidase in Enzymatic Activities at Low pH
김범수, 김윤영, 장지연, 고홍섭,
소속 상세정보
김범수 ( Kim Bum-Soo ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
김윤영 ( Kim Yoon-Young ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
장지연 ( Chang Ji-Youn ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
고홍섭 ( Kho Hong-Seop ) - Seoul National University School of Dentistry Department of Oral Medicine and Oral Diagnosis
KMID : 0355220140390040127
Abstract
Purpose: Many substances in saliva or oral health care products interact with each other. The aim of this study was to investigate interactions between hyaluronic acid (HA), lysozyme, peroxidase, and glucose oxidase (GO) in enzymatic activities at low pH levels.
Methods: HA (0.5 mg/mL), hen egg-white lysozyme (HEWL, 30 μg/mL), bovine lactoperoxidase (bLPO, 25 μg/mL), and GO (50 μg/mL) were used. The influences of HA, bLPO, and GO on HEWL activity were determined by measuring the turbidity of a Micrococcus lysodeikticus suspension. The influences of HA and HEWL on bLPO activity were determined by the NbsSCN assay, measuring the rate of oxidation of 5-thio-2-nitrobenzoic acid (Nbs) to 5,5´-dithiobis(2- nitrobenzoic acid) (Nbs)2. The influences of HA and HEWL on GO activity were determined by measuring oxidized o-dianisidine production. All experiments were performed at pH 4, 5, and 6.
Results: HA and GO did not affect the enzymatic activity of HEWL at pH 4, 5, and 6. bLPO enhanced the enzymatic activity of HEWL at pH 5 (p<0.05) and pH 6 (p<0.05) significantly. The enzymatic activity of bLPO was not affected by HA and HEWL at pH 4, 5, and 6. HA and HEWL did not affect the enzymatic activity of the GO at pH 4, 5, and 6.
Conclusions: Peroxidase enhances lysozyme activity at low pH, otherwise there were no significant interactions in enzymatic activities between HA, lysozyme, peroxidase, and GO at low pH levels.
키워드
Glucose oxidase; Hyaluronic acid; Low pH; Lysozyme; Peroxidase
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